Rapid Sequencing Kit
This kit is recommended for users who:
- require a short preparation time
- have limited access to laboratory equipment.
The Rapid Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.
Key features:
Prep time | 10 minutes |
Input amount | 400 ng HMW gDNA |
Read length | Random distribution, dependent on input fragment length |
Typical throughput | 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION |
Workflow
The Rapid Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.
