Rapid Barcoding Kit
This kit is recommended for users who:
- wish to multiplex samples to reduce price per sample
- need a PCR-free method of multiplexing to preserve additional information such as base modifications
- require a short preparation time
- have limited access to laboratory equipment.
Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow cell.
Key features:
Prep time | 10 minutes |
Input amount | 400 ng HMW gDNA |
Read length | Random distribution, dependent on input fragment length |
Typical throughput | 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION |
Workflow
The Rapid Barcoding Kit generates barcoded sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches barcoded tags to the cleaved ends: there are 12 unique barcoded tags in the kit. Barcoded samples are pooled and Rapid Sequencing Adapters are then added to the tagged ends.
