This kit is highly recommended for users who:

• have a limited amount of input material
• want to optimise their sequencing experiment for throughput
• would like to identify and quantify full-length transcripts
• are interested in differential gene expression
• want to characterise and quantify isoforms, splice variants and fusion transcripts

Key features:

Workflow

Taking full-length PolyA⁺ RNA, complementary strand synthesis and strand switching are performed using kit-supplied oligonucleotides. dscDNA is then generated by PCR amplification using primers that contain 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters.