The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 96 unique barcodes in the kit. There are 96 unique barcodes and enough of the required adapters to make 10 libraries per barcode. The barcodes have been carefully designed and extensively purified to minimise the opportunity for cross-talk.

There are currently three protocols for this kit which are provided as examples of how the PCR Barcoding Expansion 1-96 could be applied for genomic, amplicon, or cDNA libraries. Please note that if you are barcoding cDNA or amplicons, these have to be pre-made in advance. These protocols are open for adaptation and optimisation for specific experimental requirements.

This kit is used with the Ligation Sequencing Kit for library preparation.

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.