Ligation Sequencing Kit
Ligation Sequencing Kit
This kit is recommended for users who:
- want to optimise their sequencing experiment for throughput
- require control over read length
- would like to utilise upstream processes such as size selection or whole genome amplification.
Key features:
Prep time | 60 minutes minutes |
Input amount | 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR |
Read length | = fragment length |
Typical throughput | 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION |
Workflow
The Ligation Sequencing Kit offers a flexible method of preparing sequencing libraries from dsDNA (e.g. gDNA, cDNA or amplicons). The library preparation method is straightforward: DNA ends are FFPE repaired and end-prepped/dA-tailed using the NEBNext End Repair/dA-tailing module, and then sequencing adapters, supplied in the kit, are ligated onto the prepared ends.
