Field Sequencing Kit

This kit is recommended for users who:

  • are working in the field, with limited access to cold storage and laboratory equipment.

The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.

Key features:

Prep time

10 minutes

Input amount

400 ng dsDNA

Read length

Random distribution, dependent on input fragment length

Typical throughput

1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

Workflow

The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.