Direct RNA Sequencing Kit
This kit is highly recommended for users who:
- would like to remove RT or PCR bias
- have transcripts that are difficult to reverse transcribe
- are exploring attributes of native RNA such as modified bases
Key features:
Prep time | 115 minutes |
Input amount | 500 ng RNA (poly-A+) |
Read length | = RNA length |
Typical throughput | <1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION; <5 Gb in 6 hours, 5-20 Gb in 48 hours per flow cell on PromethION
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Workflow
The Direct RNA Sequencing kit (SQK-RNA002) is used to prepare any RNA with a 3’ polyA tail for 1D sequencing on the Oxford Nanopore sequencing devices. Possible input RNA includes eukaryotic mRNA, viral RNA with a polyA tail, or any RNA prepared with a polyA-tailing kit. For RNA without a polyA tail, users can follow simple instructions to design their own custom adapter to ligate a specific terminal 3’ sequence like the 3’ of tRNA or 16S rRNA. The kit requires 500 ng of input RNA.
