A Flongle Flow Cell (R9.4.1) is a consumable used with the Flongle adapter and MinION and GridION devices. It contains the proprietary sensor array and R9.4.1 nanopores suitable for all 1D experiments.

How it works:

A user simply adds their prepared library to the flow cell and starts their sequencing experiment.

Data is accumulated over time as DNA is sequenced by the nanopores. Individual read files are generated and are immediately available for analysis.

As data is streamed in real-time, a user can choose to stop a run as soon as sufficient data is gathered. A flow cell contains sufficient buffer to run for ~24 hours (under optimal conditions).

Workflow

Flongle Flow Cells are provided in a sealed pouch and are shipped at constant temperature.

It is recommended that they are kept, unopened, at 2-8° C.

Prior to use, the flow cell can be checked by performing a “Flow Cell Check” script using MinKNOW (the MinION and GridION device software). The Flow Cell Check will report the number of nanopores available for sequencing.

Once the Flow Cell Check has been performed satisfactorily, the flow cell is ready to be used for experiments. The exact protocol and reagent kits used will depend on the experiment being run.