How it works:

A user simply adds their prepared library to the flow cell and starts their sequencing experiment.

Data is accumulated over time as DNA or RNA is sequenced by the nanopores. Individual read files are generated and are immediately available for analysis.

As data is streamed in real-time, a user can choose to stop a run as soon as sufficient data is gathered. A flow cell contains sufficient buffer to run for ~64 hours (under optimal conditions) and a user can choose to run continuously or run, stop, wash, and load a new sample until the buffer and nanopores are exhausted.

Workflow

Prior to use, a Flow Cell Check should be run on the flow cell. This checks the environment for the flow cell chemistry has been maintained during shipping.

PromethION Flow Cells are provided in a sealed pouch and are shipped at a constant temperature. For long-term storage, it is recommended that they are kept in an environment not prone to large temperature fluctuations. The flow cells should be stored flat.

Once the Flow Cell Check has been performed satisfactorily, the flow cell is ready to be used for experiments. The exact protocol and reagent kits used will depend on the experiment being run.

Please return your flow cells to Oxford Nanopore Technologies after use.