DSP enables morphology-driven, high-plex profiling on a single FFPE or fresh frozen tissue section. DSP technology combines standard immunofluorescence techniques with nCounter optical barcoding quantification. The system specifications are as follows:

1- Throughput: >10 sections per day

2- Multi-analyte: RNA and Protein

3- Single Cell Segment Resolution

4- Minimum Segment Size Recommendation:

• Target detection is dependent on the size and number of cells in segment and expression level
• For small cells (e.g., T cells or B cells): 10-20 cells for protein, 50-200 cells for RNA
• For large cells (e.g., astrocytes Neurons): 1-5 cells for protein and 5-20 RNA

5- Multiplexing capability:

• 96 targets with nCounter readout
• 20000+ targets with NGS readout

6- Quantitative: 6 logs of dynamic range 

GeoMx DSP ROI Selection

The DSP software enables the user tremendeous flexibility in the ROI selection strategy to profile tissue. In particular, highlighted below are five unique profiling modalities designed to interrogate tissue samples.

The segmentation and cell-type specific approaches and achieved through the use of masks in softwre based on the fluorescent marker staining of the tissue (e.g. PanCK+ vs. PanCK-). UV light then specifically targets the ROI withmultiple illumination events, enabling the separate profiling of complex and interdigitated cell populations within a selected ROI (e.g. Tumor vs. stroma)